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Vector Labelling and Immunodetection Reagents

Economic and Reliable Antigen Unmasking

If Antigen Unmasking is a problem for you; Vector has two solutions! Vector can offer two proprietory… [more]

Economic and Reliable Antigen Unmasking Economic and Reliable Antigen Unmasking

DyLight® 594 GSL I – B4 Lectin

DyLight® Lectin For Staining Mouse Endothelial Cells DyLight® 594 Griffonia Simplicifolia Lectin I… [more]

DyLight® 594 GSL I – B4 Lectin DyLight® 594 GSL I – B4 Lectin

Multiple Antigen Labelling

The preferred method for double, triple or multiple labelling involves sequential staining of each primary… [more]

Multiple Antigen Labelling Multiple Antigen Labelling

Peroxidase Substrates and Immunoenzymatic Staining Methods

ImmPACT™ DAB Substrate Peroxidase Substrates: Vector offers a choice Successfully labelling antigens… [more]

Peroxidase Substrates and Immunoenzymatic Staining Methods Peroxidase Substrates and Immunoenzymatic Staining Methods

In Situ Hybridization Anti-DIG (digoxigenin /digoxin) Antibodies

Anti-Digoxigenin / Digoxin (DIG) in situ hybridization

Anti-DIG (digoxigenin /digoxin) Antibodies For In Situ Hybridization

Detection of Digoxigenin-Labelled Probes

In situ hybridization (ISH) probes are frequently labelled with digoxigenin (DIG), a steroid found in plants. This is particularly useful in double label applications with a biotin-labelled probe. Digoxigenin labelled probes can be detected using our high affinity, purified antibody raised against digoxigenin/digoxin. Probe detection can now be achieved using DyLight® labelled anti-digoxigenin/digoxin.

Cross-reactivity of secondary antibody in rodent models

Cross-reactivity with endogenous Ig

Eliminating background when using mouse antibodies on rat tissue or rat antibodies on mouse tissue can be an unexpected but easily solvable problem. Due to the closeness of rodent genetic structure there are similarities between antibody reactivities across the species. Thus, an anti-rat immunoglobulin used as a secondary antibody will cross-react with endogenous mouse immunoglobulins in mouse tissue and vice versa.

The ImmPRESS™ non-biotin, one-step peroxidase polymer detection system is ideal for use in rodent models…