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Vector Labelling and Immunodetection Reagents
In Situ Hybridization Anti-DIG (digoxigenin /digoxin) Antibodies
Anti-DIG (digoxigenin /digoxin) Antibodies For In Situ Hybridization Detection of Digoxigenin-Labelled Probes In situ hybridization (ISH) probes are frequently labelled with digoxigenin (DIG), a steroid found in plants. This is particularly useful in double label applications with a biotin-labelled probe. Digoxigenin labelled probes can be detected using our high affinity, purified antibody raised against digoxigenin/digoxin. Probe detection can now be achieved using DyLight® labelled anti-digoxigenin/digoxin. Cross-reactivity of secondary antibody in rodent models
Eliminating background when using mouse antibodies on rat tissue or rat antibodies on mouse tissue can be an unexpected but easily solvable problem. Due to the closeness of rodent genetic structure there are similarities between antibody reactivities across the species. Thus, an anti-rat immunoglobulin used as a secondary antibody will cross-react with endogenous mouse immunoglobulins in mouse tissue and vice versa. The ImmPRESS™ non-biotin, one-step peroxidase polymer detection system is ideal for use in rodent models… |